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KMID : 0385219980080020052
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Korean Journal of Gerontology
1998 Volume.8 No. 2 p.52 ~ p.56
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Mg2+ Dependent Endonuclease is Upregulated in Androgen Dependent LNCaP Prostate Cancer Cell Paralleling its Apoptotic Elimination
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Kang Hyeong
Kang Sang-Wook
Park In-Sun
Chun Boe-Gwun
Min Bon-Hong
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Abstract
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The effect of antiandrogen such as flutamide on the proliferative capacity of androgen sensitive LNCaP human prostate tumor cells in culture was studied. When cell growth was quantified by the colorimetric MTT assay, we found that flutamide exerted a potent dose and time-dependent inhibitory effect on LNCaP cell proliferation. Further, LDH activity in the culture media of LNCaP cells treated with flutamide was increased, suggesting that flutamide has the cytotoxic effect on LNCaP cells. We also found that flutamide induced DNA fragmentation, one of the biochemical features of apoptosis, following exposure to 80 μM of flutamide for 48 hr. Finasteride did not induce the DNA fragmentation, although it inhibited the proliferation of LNCaP cells. This result indicates that flutamide inhibited the proliferation of LNCaP cells via induction of apoptosis. Activity of Mg2+ dependent endonuclease is markedly increased in apoptotic nuclei of LNCap cells treated with flutamide. Therefore, our data demonstrate that besides DNase I or Ca2+/Mg2+ dependent endonucleases, Mg2+ dependent endonuclease is also responsible for apoptotic chromatin degradation.
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KEYWORD
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Endonuclease Flutamide, LNCaP, Apoptosis
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